PrimePCR™ SYBR® Green Assay: PRAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053593

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046332
Assay Design:   exonic
Chromosome Location:   10:135165523-135165624question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000524918 ENST00000313028 ENST00000525773 ENST00000565818 ENST00000568154 ENST00000562335 ENST00000354313 ENST00000556603 ENST00000555495 ENST00000267615 ENST00000554999 ENST00000334186 ENST00000602351 ENST00000602848 ENST00000602897 ENST00000415757 ENST00000389232 ENST00000346178 ENST00000356638 ENST00000534779 ENST00000540933 ENST00000368554 ENST00000458230 ENST00000433452 ENST00000423766

Gene Information

Description Not Available

Gene Symbol:   PRAP1
Gene Name:   proline-rich acidic protein 1
Aliases:   MGC126792, PRO1195, RP11-122K13.6, UPA
RefSeq:   NC_000010.10 NT_008818.16
Ensembl:   ENSG00000165828
Entrez:   118471
UniGene:   Hs.15951
Chromosome Mapping:   10q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.510000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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