This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053325
PrimePCR™ PreAmp for SYBR® Green Assay: ALPPL2, Human
PrimePCR™ Template for SYBR® Green Assay: ALPPL2, Human
There are at least four distinct but related alkaline phosphatases: intestinal placental placental-like and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme localized to testis thymus and certain germ cell tumors that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.