PrimePCR™ SYBR® Green Assay: ARHGAP25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053319

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGAP25, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046058
Assay Design:   exonic
Chromosome Location:   2:69034469-69034561question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000409202 ENST00000467265 ENST00000409030 ENST00000409220 ENST00000497079 ENST00000497259 ENST00000295381 ENST00000544262

Gene Information

ARHGAPs such as ARHGAP25 encode negative regulators of Rho GTPases (see ARHA; MIM 165390) which are implicated in actin remodeling cell polarity and cell migration (Katoh and Katoh 2004 [PubMed 15254788]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP25
Gene Name:   Rho GTPase activating protein 25
Aliases:   KAIA0053
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000163219
Entrez:   9938
Chromosome Mapping:   2p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.690000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download