PrimePCR™ SYBR® Green Assay: ARHGAP25, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053319

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046058
Assay Design:   exonic
Chromosome Location:   2:69034469-69034561question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000409202 ENST00000467265 ENST00000409030 ENST00000409220 ENST00000497079 ENST00000497259 ENST00000295381 ENST00000544262

Gene Information

ARHGAPs such as ARHGAP25 encode negative regulators of Rho GTPases (see ARHA; MIM 165390) which are implicated in actin remodeling cell polarity and cell migration (Katoh and Katoh 2004 [PubMed 15254788]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP25
Gene Name:   Rho GTPase activating protein 25
Aliases:   KAIA0053
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000163219
Entrez:   9938
UniGene:   Hs.531807
Chromosome Mapping:   2p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.690000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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