PrimePCR™ SYBR® Green Assay: LAPTM5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053247

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045986
Assay Design:   exonic
Chromosome Location:   1:31206367-31206468question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000294507

Gene Information

This gene encodes a transmembrane receptor that is associated with lysosomes. The encoded protein also known as E3 protein may play a role in hematopoiesis. [provided by RefSeq Feb 2009]

Gene Symbol:   LAPTM5
Gene Name:   lysosomal protein transmembrane 5
Aliases:   CLAST6, FLJ61683, FLJ97251, MGC125860, MGC125861
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000162511
Entrez:   7805
UniGene:   Hs.371021
Chromosome Mapping:   1p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995000
y-intercept 36.070000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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