PrimePCR™ SYBR® Green Assay: ZNF385A, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053202

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045941
Assay Design:   exonic
Chromosome Location:   12:54769632-54769721question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000551109 ENST00000352268 ENST00000394313 ENST00000338010 ENST00000546970 ENST00000551771 ENST00000546919 ENST00000549937 ENST00000547210 ENST00000550774

Gene Information

Zinc finger proteins such as ZNF385A are regulatory proteins that act as transcription factors bind single- or double-stranded RNA or interact with other proteins (Sharma et al. 2004 [PubMed 15527981]).[supplied by OMIM Oct 2008]

Gene Symbol:   ZNF385A
Gene Name:   zinc finger protein 385A
Aliases:   DKFZp586G1122, HZF, RZF, ZFP385, ZNF385
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000161642
Entrez:   25946
UniGene:   Hs.505653
Chromosome Mapping:   12q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.050000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.