PrimePCR™ SYBR® Green Assay: VPS28, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053183

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045922
Assay Design:   exonic
Chromosome Location:   8:145649642-145650180question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000529182 ENST00000526054 ENST00000533806 ENST00000531032 ENST00000292510 ENST00000377348 ENST00000371984 ENST00000396420 ENST00000565984 ENST00000562786 ENST00000569041 ENST00000393567 ENST00000278836 ENST00000265460 ENST00000265453 ENST00000325239 ENST00000413659 ENST00000449103 ENST00000359337 ENST00000268699 ENST00000299441 ENST00000468090 ENST00000291565 ENST00000467908 ENST00000511685 ENST00000406950 ENST00000417257 ENST00000402883 ENST00000449926 ENST00000484674 ENST00000407120 ENST

Gene Information

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23 also known as tumor susceptibility gene 101 (TSG101) and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS28
Gene Name:   vacuolar protein sorting 28 homolog (S. cerevisiae)
Aliases:   MGC60323
RefSeq:   NC_000008.10 NT_037704.5 NW_003315924.1
Ensembl:   ENSG00000160948
Entrez:   51160
UniGene:   Hs.418175
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.540000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download