PrimePCR™ SYBR® Green Assay: NLRX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053166

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NLRX1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NLRX1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045905
Assay Design:   exonic
Chromosome Location:   11:119044253-119044354question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000392557 ENST00000379937 ENST00000546474 ENST00000446501 ENST00000327359 ENST00000440513 ENST00000454811 ENST00000449394 ENST00000422249 ENST00000409991 ENST00000292199 ENST00000409109 ENST00000525863 ENST00000409265

Gene Information

This gene encodes a member of the NLR family. Alternative splicing has been observed at this gene locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   NLRX1
Gene Name:   NLR family member X1
Aliases:   CLR11.3, DLNB26, FLJ21478, MGC131937, MGC21025, NOD26, NOD5, NOD9
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000160703
Entrez:   79671
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.530000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download