PrimePCR™ SYBR® Green Assay: CLDND2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053152

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CLDND2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CLDND2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045891
Assay Design:   exonic
Chromosome Location:   19:51871717-51871816question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000291715 ENST00000601435 ENST00000357037 ENST00000434912 ENST00000358039 ENST00000360743 ENST00000340738 ENST00000554301 ENST00000555445 ENST00000427855 ENST00000381166 ENST00000299441 ENST00000308251 ENST00000251108 ENST00000444959 ENST00000221419

Gene Information

Description Not Available

Gene Symbol:   CLDND2
Gene Name:   claudin domain containing 2
Aliases:   MGC33839
RefSeq:   NC_000019.9 NG_007115.1 NT_011109.16
Ensembl:   ENSG00000160318
Entrez:   125875
Chromosome Mapping:   19q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.560000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download