PrimePCR™ SYBR® Green Assay: NPR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053130

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NPR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NPR2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045869
Assay Design:   exonic
Chromosome Location:   9:35806122-35806398question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000342694 ENST00000421267 ENST00000447210

Gene Information

This gene encodes natriuretic peptide receptor B one of two integral membrane receptors for natriuretic peptides. Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain a single membrane-spanning region and intracellularly a protein kinase homology domain a helical hinge region involved in oligomerization and a carboxyl-terminal guanylyl cyclase catalytic domain. The protein is the primary receptor for C-type natriuretic peptide (CNP) which upon ligand binding exhibits greatly increased guanylyl cyclase activity. Mutations in this gene are the cause of acromesomelic dysplasia Maroteaux type. [provided by RefSeq Jul 2008]

Gene Symbol:   NPR2
Gene Name:   natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B)
Aliases:   AMDM, ANPRB, ANPb, GUC2B, GUCY2B, NPRB, NPRBi
RefSeq:   NC_000009.11 NG_009249.1 NT_008413.18
Ensembl:   ENSG00000159899
Entrez:   4882
Chromosome Mapping:   9p21-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.660000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download