PrimePCR™ SYBR® Green Assay: LCA5L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053040

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LCA5L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LCA5L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045779
Assay Design:   exonic
Chromosome Location:   21:40800230-40800345question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000252599 ENST00000597147 ENST00000288350 ENST00000358268 ENST00000418018 ENST00000448288 ENST00000434281 ENST00000438404 ENST00000411566 ENST00000415863 ENST00000426783 ENST00000380671 ENST00000265843 ENST00000525344 ENST00000526312 ENST00000533052 ENST00000428840 ENST00000443411

Gene Information

Description Not Available

Gene Symbol:   LCA5L
Gene Name:   Leber congenital amaurosis 5-like
Aliases:   C21orf13, MGC33295
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000157578
Entrez:   150082
Chromosome Mapping:   21q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.510000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download