PrimePCR™ SYBR® Green Assay: TRIM74, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052940

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM74, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIM74, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045679
Assay Design:   exonic
Chromosome Location:   7:72430090-72430232question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000340413 ENST00000367403 ENST00000450434 ENST00000437796 ENST00000323819 ENST00000447409 ENST00000395244 ENST00000285805 ENST00000437048 ENST00000325881 ENST00000571453 ENST00000573986 ENST00000571929 ENST00000577020 ENST00000573293 ENST00000572315

Gene Information

Description Not Available

Gene Symbol:   TRIM74
Gene Name:   tripartite motif containing 74
Aliases:   MGC45440, TRIM50C
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000155428
Entrez:   378108
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 35.450000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download