PrimePCR™ SYBR® Green Assay: RBMS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052859

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045598
Assay Design:   exonic
Chromosome Location:   2:161131187-161132158question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000348849 ENST00000409075 ENST00000409289 ENST00000409972 ENST00000392753

Gene Information

This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs RNP1 and RNP2 originally described in RNA binding proteins and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication gene transcription cell cycle progression and apoptosis. Several transcript variants resulting from alternative splicing and encoding different isoforms have been described. A pseudogene for this locus is found on chromosome 12. [provided by RefSeq Feb 2009]

Gene Symbol:   RBMS1
Gene Name:   RNA binding motif, single stranded interacting protein 1
Aliases:   C2orf12, DKFZp564H0764, HCC-4, MGC15146, MGC3331, MGC70597, MGC97258, MGC97270, MGC97282, MGC99543, MSSP, MSSP-1, MSSP-2, MSSP-3, SCR2, YC1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000153250
Entrez:   5937
Chromosome Mapping:   2q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.900000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download