PrimePCR™ SYBR® Green Assay: KCNA6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052760

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KCNA6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KCNA6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045499
Assay Design:   exonic
Chromosome Location:   12:4920678-4920823question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000280684 ENST00000433855

Gene Information

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release heart rate insulin secretion neuronal excitability epithelial electrolyte transport smooth muscle contraction and cell volume. Four sequence-related potassium channel genes - shaker shaw shab and shal - have been identified in Drosophila and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel voltage-gated shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class. The coding region of this gene is intronless and the gene is clustered with genes KCNA1 and KCNA5 on chromosome 12. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNA6
Gene Name:   potassium voltage-gated channel, shaker-related subfamily, member 6
Aliases:   FLJ25134, HBK2, KV1.6
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000151079
Entrez:   3742
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.020000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download