PrimePCR™ SYBR® Green Assay: CACNA2D4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052758

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CACNA2D4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CACNA2D4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045497
Assay Design:   exonic
Chromosome Location:   12:1904465-1904848question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000585708 ENST00000586184 ENST00000536846 ENST00000538027 ENST00000588077 ENST00000587995 ENST00000538450 ENST00000382722 ENST00000314797 ENST00000397354 ENST00000378455 ENST00000378444 ENST00000406200 ENST00000342274 ENST00000257261 ENST00000403059 ENST00000344956 ENST00000275884 ENST00000496613 ENST00000492720 ENST00000537639

Gene Information

This gene encodes a member of the alpha-2/delta subunit family a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1 alpha-2/delta beta and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   CACNA2D4
Gene Name:   calcium channel, voltage-dependent, alpha 2/delta subunit 4
Aliases:   RCD4
RefSeq:   NC_000012.11 NG_012663.1 NT_009759.16
Ensembl:   ENSG00000151062
Entrez:   93589
Chromosome Mapping:   12p13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download