PrimePCR™ SYBR® Green Assay: MPP7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052735

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MPP7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MPP7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045474
Assay Design:   exonic
Chromosome Location:   10:28527513-28527662question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000375732 ENST00000375719 ENST00000441595 ENST00000337532 ENST00000540098 ENST00000445954

Gene Information

Membrane-associated guanylate kinases (MAGUKs) are important adaptor proteins involved in the assembly of protein complexes at sites of cell-cell contact. They are found in synapses adherens junctions and tight junctions. All MAGUKs contain at least 1 PDZ domain an SH3 domain and a GUK domain and many contain 1 or 2 L27 domains which are involved in multimerization of MAGUKs. MPP7 belongs to the p55 stardust subfamily of MAGUKs which is named for a Drosophila gene required for establishment of cell polarity in the developing fly embryo (Bohl et al. 2007 [PubMed 17237226]).[supplied by OMIM Mar 2008]

Gene Symbol:   MPP7
Gene Name:   membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
Aliases:   FLJ32798
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000150054
Entrez:   143098
Chromosome Mapping:   10p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 36.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download