PrimePCR™ SYBR® Green Assay: ZEB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052679

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZEB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZEB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045418
Assay Design:   exonic
Chromosome Location:   10:31799708-31803532question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000488625 ENST00000542879 ENST00000561304 ENST00000320985 ENST00000559858 ENST00000560721 ENST00000558440 ENST00000424869 ENST00000437844 ENST00000542815 ENST00000558863 ENST00000561212 ENST00000558655 ENST00000561061 ENST00000559496 ENST00000559476 ENST00000557827 ENST00000361642 ENST00000446923

Gene Information

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq Mar 2010]

Gene Symbol:   ZEB1
Gene Name:   zinc finger E-box binding homeobox 1
Aliases:   AREB6, BZP, DELTAEF1, FECD6, MGC133261, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
RefSeq:   NC_000010.10 NT_008705.16 NG_017048.1
Ensembl:   ENSG00000148516
Entrez:   6935
Chromosome Mapping:   10p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download