PrimePCR™ SYBR® Green Assay: HMCN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052670

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HMCN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HMCN2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045409
Assay Design:   exonic
Chromosome Location:   9:133233230-133233358question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000426320 ENST00000427822 ENST00000550885 ENST00000547213 ENST00000551378 ENST00000551244 ENST00000452945 ENST00000426138 ENST00000546535 ENST00000550441 ENST00000552891 ENST00000549227 ENST00000453588 ENST00000436456 ENST00000552621 ENST00000547770 ENST00000550562 ENST00000548464 ENST00000383275 ENST00000451115 ENST00000549168 ENST00000549776 ENST00000551254 ENST00000547328 ENST00000449037 ENST00000456918 ENST00000547155 ENST00000552517 ENST00000547651 ENST00000549758 ENST00000420499 ENST

Gene Information

Description Not Available

Gene Symbol:   HMCN2
Gene Name:   hemicentin 2
Aliases:   DKFZp434P0216, FLJ23816, FLJ46139, FLJ46844
RefSeq:   NC_000009.11 NT_035014.4
Ensembl:   ENSG00000148357
Entrez:   256158
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.900000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download