PrimePCR™ SYBR® Green Assay: SYBU, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052644

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SYBU, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SYBU, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045383
Assay Design:   exonic
Chromosome Location:   8:110631093-110631190question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000533895 ENST00000424158 ENST00000532779 ENST00000399066 ENST00000446070 ENST00000528331 ENST00000276646 ENST00000528647 ENST00000533065 ENST00000533171 ENST00000528045 ENST00000529190 ENST00000528569 ENST00000532189 ENST00000533821 ENST00000530841 ENST00000531230 ENST00000534501 ENST00000524720 ENST00000422135 ENST00000419099 ENST00000433638 ENST00000408908 ENST00000440310 ENST00000408889

Gene Information

Syntabulin/GOLSYN is part of a kinesin motor-adaptor complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development (Cai et al. 2007 [PubMed 17611281]).[supplied by OMIM Mar 2008]

Gene Symbol:   SYBU
Gene Name:   syntabulin (syntaxin-interacting)
Aliases:   FLJ20366, GOLSYN, KIAA1472, OCSYN, SNPHL
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000147642
Entrez:   55638
Chromosome Mapping:   8q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download