PrimePCR™ SYBR® Green Assay: RNF145, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052554

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RNF145, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RNF145, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045293
Assay Design:   exonic
Chromosome Location:   5:158585554-158585701question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000274542 ENST00000519865 ENST00000424310 ENST00000521606 ENST00000518802 ENST00000520638

Gene Information

Description Not Available

Gene Symbol:   RNF145
Gene Name:   ring finger protein 145
Aliases:   DKFZp686M11215, FLJ31951, FLJ44310
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000145860
Entrez:   153830
Chromosome Mapping:   5q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.510000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download