PrimePCR™ SYBR® Green Assay: REN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052425

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: REN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: REN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045164
Assay Design:   exonic
Chromosome Location:   1:204123969-204124078question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000272190 ENST00000367195

Gene Information

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone releasevasoconstriction and increase in blood pressure. Renin an aspartyl protease cleaves angiotensinogen to form angiotensin I which is converted to angiotensin II by angiotensin I converting enzyme an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq Jul 2008]

Gene Symbol:   REN
Gene Name:   renin
Aliases:   FLJ10761, HNFJ2
RefSeq:   NC_000001.10 NG_012122.1 NT_004487.19
Ensembl:   ENSG00000143839
Entrez:   5972
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download