PrimePCR™ SYBR® Green Assay: ZNF787, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052318

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ZNF787, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF787, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045056
Assay Design:   exonic
Chromosome Location:   19:56600440-56632545question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000371884 ENST00000294339 ENST00000371883 ENST00000448023 ENST00000359128 ENST00000301749 ENST00000419350 ENST00000270459 ENST00000586787 ENST00000587279 ENST00000529064 ENST00000425753 ENST00000529048 ENST00000584601 ENST00000578199 ENST00000584450 ENST00000269571 ENST00000578709 ENST00000584099 ENST00000541774 ENST00000406381 ENST00000540147 ENST00000540042 ENST00000445067 ENST00000369744 ENST00000466741 ENST00000524472 ENST00000497220 ENST00000369748 ENST00000343257 ENST00000428304 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF787
Gene Name:   zinc finger protein 787
Aliases:   TIP20
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000142409
Entrez:   126208
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.440000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download