PrimePCR™ SYBR® Green Assay: VPS53, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052262

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VPS53, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VPS53, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045000
Assay Design:   exonic
Chromosome Location:   17:489603-505135question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000437048 ENST00000291074 ENST00000571805 ENST00000401468 ENST00000570650 ENST00000446250

Gene Information

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS53
Gene Name:   vacuolar protein sorting 53 homolog (S. cerevisiae)
Aliases:   FLJ10979, FLJ41112, FLJ61757, HCCS1, MGC39512, hVps53L, pp13624
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000141252
Entrez:   55275
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.770000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download