PrimePCR™ SYBR® Green Assay: CDH11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052246

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CDH11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CDH11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044984
Assay Design:   exonic
Chromosome Location:   16:65038751-65038893question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000394156 ENST00000268603 ENST00000567934 ENST00000562998 ENST00000564317 ENST00000562712 ENST00000562882 ENST00000562325

Gene Information

This gene encodes a type II classical cadherin from the cadherin superfamily integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain a single membrane-spanning domain and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines and its upregulation during differentiation suggests a specific function in bone development and maintenance. [provided by RefSeq Jul 2008]

Gene Symbol:   CDH11
Gene Name:   cadherin 11, type 2, OB-cadherin (osteoblast)
Aliases:   CAD11, CDHOB, OB, OSF-4
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140937
Entrez:   1009
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.130000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download