PrimePCR™ SYBR® Green Assay: SRP14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052217

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044955
Assay Design:   exonic
Chromosome Location:   15:40328494-40328684question
Amplicon Length:   161
Splice Variants Targeted:   ENST00000267884 ENST00000558720 ENST00000560773

Gene Information

Description Not Available

Gene Symbol:   SRP14
Gene Name:   signal recognition particle 14kDa (homologous Alu RNA binding protein)
Aliases:   ALURBP, MGC14326
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000140319
Entrez:   6727
UniGene:   Hs.533732
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.240000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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