PrimePCR™ SYBR® Green Assay: SSB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052098

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SSB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SSB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044836
Assay Design:   exonic
Chromosome Location:   2:170662064-170662291question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000334746 ENST00000554565 ENST00000553975 ENST00000393170 ENST00000422006 ENST00000260956 ENST00000417292 ENST00000409333

Gene Information

The protein encoded by this gene is involved in diverse aspects of RNA metabolism including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion processing 5' and 3' ends of pre-tRNA precursors acting as an RNA chaperone and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. [provided by RefSeq Nov 2011]

Gene Symbol:   SSB
Gene Name:   Sjogren syndrome antigen B (autoantigen La)
Aliases:   LARP3, La
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000138385
Entrez:   6741
Chromosome Mapping:   2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download