PrimePCR™ SYBR® Green Assay: TMPRSS4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052035

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044773
Assay Design:   exonic
Chromosome Location:   11:117973966-117975522question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000534111 ENST00000522307 ENST00000523251 ENST00000437212 ENST00000522824 ENST00000522151 ENST00000517544

Gene Information

This gene encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes whose malfunction often leads to human diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellular region containing the serine protease domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   TMPRSS4
Gene Name:   transmembrane protease, serine 4
Aliases:   CAPH2, MT-SP2, TMPRSS3
RefSeq:   NC_000011.9 NT_033899.8 NG_011858.2
Ensembl:   ENSG00000137648
Entrez:   56649
UniGene:   Hs.161985
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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