PrimePCR™ SYBR® Green Assay: DSCC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052000

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DSCC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DSCC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044738
Assay Design:   exonic
Chromosome Location:   8:120846335-120846462question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000313655

Gene Information

CHTF18 (MIM 613201) CHTF8 (MIM 613202) and DSCC1 are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al. 2003 [PubMed 12766176]; Bermudez et al. 2003 [PubMed 12930902]).[supplied by OMIM Dec 2009]

Gene Symbol:   DSCC1
Gene Name:   defective in sister chromatid cohesion 1 homolog (S. cerevisiae)
Aliases:   DCC1, MGC5528
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000136982
Entrez:   79075
Chromosome Mapping:   8q24.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.600000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download