PrimePCR™ SYBR® Green Assay: TFCP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051866

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TFCP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TFCP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044604
Assay Design:   exonic
Chromosome Location:   12:51511503-51512475question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000257915 ENST00000549867 ENST00000548115 ENST00000548108 ENST00000307660

Gene Information

This gene encodes a transcription factor that binds the alpha-globin promoter and activates transcription of the alpha-globin gene. The encoded protein regulates erythroid gene expression plays a role in the transcriptional switch of globin gene promoters and it activates many other cellular and viral gene promoters. The gene product interacts with certain inflammatory response factors and polymorphisms of this gene may be involved in the pathogenesis of Alzheimer's disease. [provided by RefSeq Mar 2010]

Gene Symbol:   TFCP2
Gene Name:   transcription factor CP2
Aliases:   LBP1C, LSF, LSF1D, SEF, TFCP2C
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135457
Entrez:   7024
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download