PrimePCR™ SYBR® Green Assay: TRAFD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051854

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRAFD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRAFD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044592
Assay Design:   exonic
Chromosome Location:   12:112572559-112572673question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000412615 ENST00000549358 ENST00000552896 ENST00000257604

Gene Information

The innate immune system confers host defense against viral and microbial infection and TRAFD1 is a negative feedback regulator that controls excessive immune responses (Sanada et al. 2008 [PubMed 18849341]).[supplied by OMIM Dec 2009]

Gene Symbol:   TRAFD1
Gene Name:   TRAF-type zinc finger domain containing 1
Aliases:   FLN29
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000135148
Entrez:   10906
Chromosome Mapping:   12q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.350000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download