PrimePCR™ SYBR® Green Assay: ZNF20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051680

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044418
Assay Design:   exonic
Chromosome Location:   19:12250995-12251106question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000253807 ENST00000409700 ENST00000600335 ENST00000334213 ENST00000418866 ENST00000358353 ENST00000397542 ENST00000514798 ENST00000549489 ENST00000475668 ENST00000509176 ENST00000432775 ENST00000333691 ENST00000379119 ENST00000563332 ENST00000399599 ENST00000567695 ENST00000564828 ENST00000285879 ENST00000406005 ENST00000228468 ENST00000447966 ENST00000552438 ENST00000377673 ENST00000538285 ENST00000548869 ENST00000369817 ENST00000569657 ENST00000563244 ENST00000597605

Gene Information

Description Not Available

Gene Symbol:   ZNF20
Gene Name:   zinc finger protein 20
Aliases:   FLJ39241, KOX13
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000132010
Entrez:   7568
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download