PrimePCR™ SYBR® Green Assay: PRR7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051631

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRR7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRR7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044369
Assay Design:   exonic
Chromosome Location:   5:176881989-176882151question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000355423 ENST00000584667 ENST00000602735 ENST00000269703 ENST00000373578 ENST00000373567 ENST00000373558 ENST00000445403 ENST00000360723 ENST00000358208 ENST00000550422 ENST00000307885 ENST00000357869 ENST00000330710 ENST00000507881 ENST00000323249 ENST00000502922 ENST00000510492 ENST00000403625 ENST00000404260 ENST00000316219 ENST00000449561 ENST00000338244 ENST00000379333 ENST00000424750 ENST00000547195 ENST00000324366 ENST00000397441 ENST00000216350 ENST00000553897 ENST00000538452 ENST

Gene Information

Description Not Available

Gene Symbol:   PRR7
Gene Name:   proline rich 7 (synaptic)
Aliases:   MGC10772
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000131188
Entrez:   80758
Chromosome Mapping:   5q35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.880000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download