PrimePCR™ SYBR® Green Assay: SLC34A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051629

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC34A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC34A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044367
Assay Design:   exonic
Chromosome Location:   5:176814805-176815015question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000398742 ENST00000334122 ENST00000569393 ENST00000512593 ENST00000324417 ENST00000295874 ENST00000474889 ENST00000388712 ENST00000388711 ENST00000373068 ENST00000373069 ENST00000373066 ENST00000432073 ENST00000373064 ENST00000433501 ENST00000380769

Gene Information

This gene encodes a member of the type II sodium-phosphate cotransporter family. Mutations in this gene are associated with hypophosphatemia nephrolithiasis/osteoporosis 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2009]

Gene Symbol:   SLC34A1
Gene Name:   solute carrier family 34 (sodium phosphate), member 1
Aliases:   FRTS2, NAPI-3, NPHLOP1, NPT2, NPTIIa, SLC11, SLC17A2
RefSeq:   NC_000005.9 NT_023133.13 NG_016223.1
Ensembl:   ENSG00000131183
Entrez:   6569
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.550000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download