PrimePCR™ SYBR® Green Assay: ARHGEF9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051623

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGEF9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGEF9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044361
Assay Design:   exonic
Chromosome Location:   X:62875495-62885779question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000253401 ENST00000374878 ENST00000374872 ENST00000437457 ENST00000374870 ENST00000433323 ENST00000601443 ENST00000594535 ENST00000601864 ENST00000602267 ENST00000598400 ENST00000597149

Gene Information

The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE) also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2010]

Gene Symbol:   ARHGEF9
Gene Name:   Cdc42 guanine nucleotide exchange factor (GEF) 9
Aliases:   COLLYBISTIN, EIEE8, HPEM-2, KIAA0424, PEM-2, PEM2
RefSeq:   NC_000023.10 NT_011669.17 NG_016975.1
Ensembl:   ENSG00000131089
Entrez:   23229
Chromosome Mapping:   Xq11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.810000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download