PrimePCR™ SYBR® Green Assay: SHBG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051520

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SHBG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SHBG, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044258
Assay Design:   exonic
Chromosome Location:   17:7534960-7535063question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000574539 ENST00000576728 ENST00000575314 ENST00000570547 ENST00000572262 ENST00000576478 ENST00000380450 ENST00000416273 ENST00000575903 ENST00000576830 ENST00000571153 ENST00000576152 ENST00000441599 ENST00000340624

Gene Information

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein binds each steroid molecule as a dimer formed from identical or nearly identical monomers. The use of alternate promoters and alternatively spliced transcripts have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2009]

Gene Symbol:   SHBG
Gene Name:   sex hormone-binding globulin
Aliases:   ABP, MGC126834, MGC138391, SBP, TEBG
RefSeq:   NG_028105.1 NC_000017.10 NG_011981.1 NT_010718.16
Ensembl:   ENSG00000129214
Entrez:   6462
Chromosome Mapping:   17p13-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download