PrimePCR™ SYBR® Green Assay: ARPP19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051503

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARPP19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARPP19, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044241
Assay Design:   exonic
Chromosome Location:   15:52844103-52844216question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000566423 ENST00000249822 ENST00000561650 ENST00000568196 ENST00000563277 ENST00000567669 ENST00000569723 ENST00000569281 ENST00000564163 ENST00000563566 ENST00000561971

Gene Information

The 19-kD cAMP-regulated phosphoprotein plays a role in regulating mitosis by inhibiting protein phosphatase-2A (PP2A; see MIM 176915) (summary by Gharbi-Ayachi et al. 2010 [PubMed 21164014]).[supplied by OMIM Feb 2011]

Gene Symbol:   ARPP19
Gene Name:   cAMP-regulated phosphoprotein, 19kDa
Aliases:   ARPP-16, ARPP-19, ARPP16, ENSAL, FLJ41622
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000128989
Entrez:   10776
Chromosome Mapping:   15q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.830000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download