PrimePCR™ SYBR® Green Assay: THOC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051356

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: THOC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: THOC2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044094
Assay Design:   exonic
Chromosome Location:   X:122747939-122753263question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000448128 ENST00000441692 ENST00000416618 ENST00000355725 ENST00000491737 ENST00000245838

Gene Information

The TREX multiprotein complex binds specifically to spliced mRNAs to facilitate mRNA export. The protein encoded by this gene is a member of the THO complex a subset of the TREX complex. The encoded protein interacts with the THOC1 protein.[provided by RefSeq Jun 2010]

Gene Symbol:   THOC2
Gene Name:   THO complex 2
Aliases:   CXorf3, THO2, dJ506G2.1, hTREX120
RefSeq:   NC_000023.10 NG_021468.1 NT_011786.16
Ensembl:   ENSG00000125676
Entrez:   57187
Chromosome Mapping:   Xq25-q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.960000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download