PrimePCR™ SYBR® Green Assay: AHNAK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051334

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AHNAK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AHNAK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044072
Assay Design:   exonic
Chromosome Location:   11:62302576-62303423question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000449771 ENST00000549151 ENST00000389212 ENST00000548919 ENST00000395358 ENST00000466322 ENST00000405493 ENST00000171000 ENST00000446957 ENST00000269554 ENST00000348261 ENST00000565831 ENST00000358590 ENST00000265074 ENST00000339042 ENST00000326958 ENST00000334011 ENST00000356865 ENST00000380548 ENST00000380545 ENST00000380538 ENST00000530124 ENST00000257247 ENST00000533365 ENST00000378024 ENST00000530285 ENST00000528508 ENST00000531324 ENST00000372315 ENST00000243964 ENST00000454036 ENST

Gene Information

Description Not Available

Gene Symbol:   AHNAK
Gene Name:   AHNAK nucleoprotein
Aliases:   AHNAKRS, MGC5395
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000124942
Entrez:   79026
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download