PrimePCR™ SYBR® Green Assay: SERPINA7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051256

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SERPINA7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SERPINA7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043994
Assay Design:   exonic
Chromosome Location:   X:105280646-105280762question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000327674 ENST00000372563

Gene Information

There are three proteins including thyroxine-binding globulin (TBG) transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 353 -triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein TBG in serum. It belongs to the serpin family in genomics but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency which has been classified as partial deficiency complete deficiency and excess based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found but the full-length nature of these variants has not been determined.[provided by RefSeq May 2010]

Gene Symbol:   SERPINA7
Gene Name:   serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Aliases:   FLJ17820, TBG
RefSeq:   NC_000023.10 NG_021252.1 NT_011651.17
Ensembl:   ENSG00000123561
Entrez:   6906
Chromosome Mapping:   Xq22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.910000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download