PrimePCR™ SYBR® Green Assay: EBPL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051239

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EBPL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EBPL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043977
Assay Design:   exonic
Chromosome Location:   13:50265396-50265587question
Amplicon Length:   162
Splice Variants Targeted:   ENST00000436010 ENST00000495273 ENST00000464233 ENST00000432854 ENST00000449237 ENST00000559488 ENST00000382102 ENST00000409991 ENST00000292199 ENST00000409109 ENST00000011653 ENST00000378284 ENST00000378272 ENST00000242827 ENST00000378270 ENST00000378268 ENST00000378282 ENST00000378274 ENST00000250373 ENST00000436396 ENST00000211998 ENST00000325509 ENST00000594128

Gene Information

Description Not Available

Gene Symbol:   EBPL
Gene Name:   emopamil binding protein-like
Aliases:   EBRP
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000123179
Entrez:   84650
Chromosome Mapping:   13q12-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download