PrimePCR™ SYBR® Green Assay: ELL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051061

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ELL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ELL2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043799
Assay Design:   exonic
Chromosome Location:   5:95297316-95297543question
Amplicon Length:   198
Splice Variants Targeted:   ENST00000381055 ENST00000525780 ENST00000326053 ENST00000237853 ENST00000513343 ENST00000431061 ENST00000338244 ENST00000379333 ENST00000465357 ENST00000539517 ENST00000265316 ENST00000295750 ENST00000439002 ENST00000360392

Gene Information

Description Not Available

Gene Symbol:   ELL2
Gene Name:   elongation factor, RNA polymerase II, 2
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000118985
Entrez:   22936
Chromosome Mapping:   5q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 35.460000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download