PrimePCR™ SYBR® Green Assay: IFT46, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051026

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: IFT46, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IFT46, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043764
Assay Design:   exonic
Chromosome Location:   11:118427631-118428472question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000264021 ENST00000264020 ENST00000530872 ENST00000531939 ENST00000534156 ENST00000534114 ENST00000528378 ENST00000533918 ENST00000597276 ENST00000598014 ENST00000595543 ENST00000600698 ENST00000601455 ENST00000598210 ENST00000598778 ENST00000595675

Gene Information

Description Not Available

Gene Symbol:   IFT46
Gene Name:   intraflagellar transport 46 homolog (Chlamydomonas)
Aliases:   C11orf2, C11orf60, FLJ21827
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000118096
Entrez:   56912
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download