PrimePCR™ SYBR® Green Assay: SLC5A7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050896

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC5A7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC5A7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043634
Assay Design:   exonic
Chromosome Location:   2:108627186-108627283question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000540517 ENST00000264047 ENST00000409059

Gene Information

Choline is a direct precursor of acetylcholine (ACh) a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic cognitive and motor functions. SLC5A7 is a Na(+)- and Cl(-)- dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons (Apparsundaram et al. 2000 [PubMed 11027560]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC5A7
Gene Name:   solute carrier family 5 (choline transporter), member 7
Aliases:   CHT, CHT1, MGC126299, MGC126300, hCHT
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000115665
Entrez:   60482
Chromosome Mapping:   2q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download