PrimePCR™ SYBR® Green Assay: UXS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050894

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: UXS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: UXS1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043632
Assay Design:   exonic
Chromosome Location:   2:106729121-106739443question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000283148 ENST00000409501 ENST00000409032 ENST00000441952 ENST00000416298 ENST00000444193 ENST00000540130 ENST00000428048 ENST00000327259 ENST00000528499 ENST00000263574 ENST00000338167 ENST00000278756 ENST00000543137 ENST00000539648 ENST00000348370 ENST00000559209 ENST00000557998 ENST00000561186 ENST00000434298 ENST00000338875 ENST00000251166 ENST00000537233 ENST00000574025 ENST00000539968 ENST00000378076

Gene Information

This gene encodes an enyzme found in the perinuclear Golgi which catalyzes the synthesis of UDP-xylose used in glycosaminoglycan (GAG) synthesis on proteoglycans. The GAG chains are covalently attached to proteoglycans which participate in signaling pathways during development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   UXS1
Gene Name:   UDP-glucuronate decarboxylase 1
Aliases:   FLJ23591, SDR6E1, UGD
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000115652
Entrez:   80146
Chromosome Mapping:   2q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download