PrimePCR™ SYBR® Green Assay: PLCH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050827

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLCH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLCH1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043565
Assay Design:   exonic
Chromosome Location:   3:155282786-155286025question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000494598 ENST00000460012 ENST00000447496 ENST00000340059 ENST00000334686 ENST00000414191

Gene Information

PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 45-bisphosphate (PtdIns(45)P2) to generate second messengers inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al. 2005 [PubMed 15702972]).[supplied by OMIM Jun 2009]

Gene Symbol:   PLCH1
Gene Name:   phospholipase C, eta 1
Aliases:   DKFZp434C1372, MGC117152, PLCL3
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114805
Entrez:   23007
Chromosome Mapping:   3q25.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.320000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download