PrimePCR™ SYBR® Green Assay: HYAL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050807

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HYAL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HYAL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043545
Assay Design:   exonic
Chromosome Location:   3:50339496-50339636question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000571551 ENST00000570967 ENST00000576782 ENST00000572970 ENST00000575445 ENST00000570958 ENST00000395144 ENST00000266031 ENST00000320295 ENST00000395143 ENST00000457214 ENST00000447605

Gene Information

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HYAL1
Gene Name:   hyaluronoglucosaminidase 1
Aliases:   HYAL-1, LUCA1, MGC45987, NAT6
RefSeq:   NC_000003.11 NT_022517.18 NG_009295.1
Ensembl:   ENSG00000114378
Entrez:   3373
Chromosome Mapping:   3p21.3-p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.530000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download