PrimePCR™ SYBR® Green Assay: SLC38A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050635

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC38A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC38A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043373
Assay Design:   exonic
Chromosome Location:   12:46633495-46633624question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000550173 ENST00000552197 ENST00000546893 ENST00000439706 ENST00000549049 ENST00000398637

Gene Information

Amino acid transporters play essential roles in the uptake of nutrients production of energy chemical metabolism detoxification and neurotransmitter cycling. SLC38A1 is an important transporter of glutamine an intermediate in the detoxification of ammonia and the production of urea. Glutamine serves as a precursor for the synaptic transmitter glutamate (Gu et al. 2001 [PubMed 11325958]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC38A1
Gene Name:   solute carrier family 38, member 1
Aliases:   ATA1, NAT2, SAT1, SNAT1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000111371
Entrez:   81539
Chromosome Mapping:   12q13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.470000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download