PrimePCR™ SYBR® Green Assay: NCS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qHsaCEP0050436

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: NCS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NCS1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043174
Assay Design:   exonic
Chromosome Location:   9:132998920-132999067question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000409271 ENST00000258443 ENST00000376651 ENST00000317446 ENST00000445819 ENST00000326972 ENST00000424053 ENST00000429201 ENST00000512583 ENST00000305107 ENST00000299106 ENST00000441717 ENST00000529443 ENST00000300651 ENST00000325239 ENST00000413659 ENST00000409009 ENST00000264448 ENST00000377715 ENST00000375856 ENST00000602795 ENST00000344018 ENST00000374839 ENST00000337990 ENST00000447788 ENST00000545618 ENST00000455584 ENST00000578237 ENST00000336708 ENST00000322927 ENST00000426788 ENST

Gene Information

This gene is a member of the neuronal calcium sensor gene family which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NCS1
Gene Name:   neuronal calcium sensor 1
Aliases:   DKFZp761L1223, FLUP, FREQ
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000107130
Entrez:   23413
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download