PrimePCR™ SYBR® Green Assay: GARS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050392

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043130
Assay Design:   exonic
Chromosome Location:   7:30656743-30656881question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000389266

Gene Information

This gene encodes glycyl-tRNA synthetase one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases polymyositis or dermatomyositis. [provided by RefSeq Jul 2008]

Gene Symbol:   GARS
Gene Name:   glycyl-tRNA synthetase
Aliases:   CMT2D, DSMAV, GlyRS, HMN5, SMAD1
RefSeq:   NC_000007.13 NG_007942.1 NT_007819.17
Ensembl:   ENSG00000106105
Entrez:   2617
UniGene:   Hs.404321
Chromosome Mapping:   7p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.790000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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