PrimePCR™ SYBR® Green Assay: GTPBP10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050359

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GTPBP10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GTPBP10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043097
Assay Design:   exonic
Chromosome Location:   7:89976080-89982220question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000417207 ENST00000222511 ENST00000257659 ENST00000450619 ENST00000426366

Gene Information

Small G proteins such as GTPBP10 act as molecular switches that play crucial roles in the regulation of fundamental cellular processes such as protein synthesis nuclear transport membrane trafficking and signal transduction (Hirano et al. 2006 [PubMed 17054726]).[supplied by OMIM Mar 2008]

Gene Symbol:   GTPBP10
Gene Name:   GTP-binding protein 10 (putative)
Aliases:   DKFZp686A10121, FLJ38242, MGC104191, ObgH2
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000105793
Entrez:   85865
Chromosome Mapping:   7q21.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.090000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download