PrimePCR™ SYBR® Green Assay: NUBP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050230

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NUBP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NUBP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042968
Assay Design:   exonic
Chromosome Location:   16:10850637-10851864question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000279024 ENST00000555572 ENST00000373381 ENST00000574334 ENST00000283027 ENST00000433392 ENST00000571175 ENST00000269142 ENST00000578121 ENST00000262316 ENST00000250160 ENST00000394196 ENST00000337975 ENST00000536947 ENST00000377981 ENST00000224721

Gene Information

NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins (Nakashima et al. 1999 [PubMed 10486206]).[supplied by OMIM Mar 2008]

Gene Symbol:   NUBP1
Gene Name:   nucleotide binding protein 1 (MinD homolog, E. coli)
Aliases:   MGC117406, MGC130052, MGC130053, NBP, NBP1
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103274
Entrez:   4682
Chromosome Mapping:   16p13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.650000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download